CRISPR/Cas9 Systems and Gene Editing Technology

Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology.

Until very recently, the prospect of introducing mutations or exogenous DNA sequences at precise locations in the genomes of plants and animals was difficult, if not impossible. This rapidly changed with the demonstration that the type II CRISPR-Cas complex, a bacterial anti-viral surveillance system, could be engineered into a simple and robust platform for introducing double-stranded DNA brea...

Genome Editing using NucleofectorTM Technology

Ethics and germline gene editing.

T he current kerfuffle around the use of CRISPR/Cas9 and other gene editing technologies in human germline research is the latest in a series of related controversies at the intersection of science, medicine, and ethics [1]. Soon after a prominent ad hoc group of scientists called for a moratorium on clinical applications of germline gene editing [2], a research group from China published an ar...

Gene Editing Versus Morphants

Kok et al. conclude that results with Morpholino oligos, which are not recapitulated by natural mutations or gene editing mutations, must be due to off-target effects and, when morphants and mutants differ, they contend that antisense oligo studies should be disregarded. Morpholinos and gene editing mutagenesis are very different technologies and, even if both work perfectly, may yield differen...

Compiler Technology for Blue Gene Systems

Standard compilers are incapable of fully harnessing the enormous performance potential of Blue Gene systems. To reach the leading position in the Top500 supercomputing list, IBM had to put considerable effort into coding and tuning a limited range of low-level numerical kernel routines by hand. In this paper the Vienna MAP compiler is presented, which particularly targets signal transform code...